LOCUS       sequence-160987-         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for inducible expression of LacZ with 
            N-terminal 3xFLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
  TITLE     Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor 
            Sequestration in Triple-Negative Breast Cancer.
  JOURNAL   Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 
            10.1016/j.devcel.2017.10.027.
  PUBMED    29161592
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.10"; journalName: "Dev Cell"; date: 
            "2017-11-20- 20"; volume: "43"; issue: "4"; pages: "418-435.e13"
COMMENT     Sequence Label: pEN_TT 3xFLAG LacZ
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..86
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(95..117)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cgtcagatcg ccactagatg gactacaaag accatgacgg tgattataaa gatcatgaca
       61 tcgattacaa ggatgacgat gacaagacta gtgtcgtttt acaacgtcgt gactgggaaa
      121 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
      181 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
      241 ggcgctttgc ctggtttccg gcaccagaag cggtgccgga aagctggctg gagtgcgatc
      301 ttcctgaggc cgatactgtc gtcgtcccct caaactggca gatgcacggt tacgatgcgc
      361 ccatctacac caacgtgacc tatcccatta cggtcaatcc gccgtttgtt cccacggaga
      421 atccgacggg ttgttactcg ctcacattta atgttgatga aagctggcta caggaaggcc
      481 agacgcgaat tatttttgat ggcgttaact cggcgtttca tctgtggtgc aacgggcgct
      541 gggtcggtta cggccaggac agtcgtttgc cgtctgaatt tgacctgagc gcatttttac
      601 gcgccggaga aaaccgcctc gcggtgatgg tgctgcgctg gagtgacggc agttatctgg
      661 aagatcagga tatgtggcgg atgagcggca ttttccgtga cgtctcgttg ctgcataaac
      721 cgactacaca a
//
