LOCUS       sequence-160988-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for inducible expression of LacZ with 
            N-terminal 3xFLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
  TITLE     Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor 
            Sequestration in Triple-Negative Breast Cancer.
  JOURNAL   Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 
            10.1016/j.devcel.2017.10.027.
  PUBMED    29161592
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.10"; journalName: "Dev Cell"; date: 
            "2017-11-20- 20"; volume: "43"; issue: "4"; pages: "418-435.e13"
COMMENT     Sequence Label: pEN_TT 3xFLAG LacZ
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    87..186
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga
       61 cacgggccag agctgcagct ggatggcaaa taatgatttt attttgactg atagtgacct
      121 gttcgttgca acaaattgat aagcaatgct ttcttataat gccaactttg tacaagaaag
      181 ctgggtctag atatctcgaa aacgcattag tcttccaatt gtcatttttg acaccagacc
      241 aactggtaat ggtagcgacc ggcgctcagc tgtaattccg ccgatactga cgggctccag
      301 gagtcgtctc caccaatccc catatggaaa ccgtcgatat tcagccatgt gccttcttcc
      361 gcgtgcagca gatggcgatg gctggtttcc atcagttgct gttgactgta gcggctgatg
      421 ttgaactgga agtcgccgcg ccactggtgt gggccataat tcaattcgcg cgtcccgcag
      481 cgcagaccgt tttcgctcgg gaagacgtac ggggtataca tgtctgacaa tggcagatcc
      541 cagcggtcaa aacaggcggc agtaaggcgg tcgggatagt tttcttgcgg ccctaatccg
      601 agccagttta cccgctctgc tacctgcgcc agctggcagt tcaggccaat ccgcgccgga
      661 tgcggtgtat cgctcgccac ttcaacatca acggtaatcg ccatttgacc actaccatca
      721 atccggtagg ttttccggct gataaataag gttttcccct gatgctgcca cgcgtgagcg
      781 gtcgtaatca gcaccgcgtc agca
//
