LOCUS       sequence-160991-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Useful for tagging yeast proteins with C-terminal YFP tag; G418 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Hailey DW, Davis TN, Muller EG
  TITLE     Fluorescence resonance energy transfer using color variants of green
            fluorescent protein.
  JOURNAL   Methods Enzymol. 2002;351:34-49.
  PUBMED    12073355
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2002"; volume: "351"; pages: "34-49"
COMMENT     Sequence Label: pDH6
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        59..77
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     59..76
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             138..851
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGLKCFARYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(165..193)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     788..808
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tattgtcgtt agaacgcggc tacaattaat acataacctt atgtatcata cacatacgat
       61 ttaggtgaca ctatagaacg cggccgccag ctgaagcttc gtacgctgca ggtcgacgga
      121 tccccgggtt aattaacagt aaaggagaag aacttttcac tggagttgtc ccaattcttg
      181 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
      241 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc
      301 catggccaac acttgtcact actttcggtt atggtctaaa atgctttgct agatacccag
      361 atcatatgaa acggcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa
      421 gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag
      481 gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca
      541 ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca
      601 aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg
      661 ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac
      721 cagacaacca ttacctgtcc tatcaatctg ccctttcgaa agatcccaac gaaaagagag
      781 accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac
      841 tatacaaata gggcgcgcca cttctaaata agcgaatttc ttatgattta tgatttt
//
