LOCUS       sequence-160993-         694 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses constitutively active human MKK6. Multiple cloning site 2 
            is empty..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Shah M, Smolko CM, Kinicki S, Chapman ZD, Brautigan DL, Janes KA
  TITLE     Profiling Subcellular Protein Phosphatase Responses to 
            Coxsackievirus B3 Infection of Cardiomyocytes.
  JOURNAL   Mol Cell Proteomics. 2017 Apr;16(4 suppl 1):S244-S262. doi: 
            10.1074/mcp.O116.063487. Epub 2017 Feb 7.
  PUBMED    28174228
REFERENCE   2  (bases 1 to 694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2017-04"; volume: "16(4 suppl 1):S244-S262. 
            doi"; pages: " 10.1074/mcp.O116.063487. Epub 2017 Feb 7"
COMMENT     Sequence Label: pCDFDuet-MKK6-EE
FEATURES             Location/Qualifiers
     source          1..694
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     97..116
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        97..115
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    116..140
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             196..213
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc
       61 gacgctctcc cttatgcgac tcctgcatta ggaaattaat acgactcact ataggggaat
      121 tgtgagcgga taacaattcc cctgtagaaa taattttgtt taactttaat aaggagatat
      181 accatgggca gcagccatca ccatcatcac cacagccagg atctatgtct cagtcgaaag
      241 gcaagaagcg aaaccctggc cttaaaattc caaaagaagc atttgaacaa cctcagacca
      301 gttccacacc acctagagat ttagactcca aggcttgcat ttctattgga aatcagaact
      361 ttgaggtgaa ggcagatgac ctggagccta taatggaact gggacgaggt gcgtacgggg
      421 tggtggagaa gatgcggcac gtgcccagcg ggcagatcat ggcagtgaag cggatccgag
      481 ccacagtaaa tagccaggaa cagaaacggc tactgatgga tttggatatt tccatgagga
      541 cggtggactg tccattcact gtcacctttt atggcgcact gtttcgggag ggtgatgtgt
      601 ggatctgcat ggagctcatg gatacatcac tagataaatt ctacaaacaa gttattgata
      661 aaggccagac aattccagag gacatcttag ggaa
//
