LOCUS       sequence-160997-         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Useful for tagging yeast proteins with C-terminal CFP tag; 
            hygromycin-B selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Hailey DW, Davis TN, Muller EG
  TITLE     Fluorescence resonance energy transfer using color variants of green
            fluorescent protein.
  JOURNAL   Methods Enzymol. 2002;351:34-49.
  PUBMED    12073355
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2002"; volume: "351"; pages: "34-49"
COMMENT     Sequence Label: pBS4
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        59..77
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     59..76
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             138..848
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAH
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(165..193)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     788..808
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tattgtcgtt agaacgcggc tacaattaat acataacctt atgtatcata cacatacgat
       61 ttaggtgaca ctatagaacg cggccgccag ctgaagcttc gtacgctgca ggtcgacgga
      121 tccccgggtt aattaacagt aaaggagaag aacttttcac tggagttgtc ccaattcttg
      181 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
      241 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc
      301 catggccaac acttgtcact actttgactt ggggtgttca atgcttttca agatacccag
      361 atcatatgaa acggcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa
      421 gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag
      481 gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca
      541 ttcttggaca caaattggaa tacaactata tttcacacaa tgtatacatc actgcagaca
      601 aacaaaagaa tggaatcaaa gctcatttca aaattagaca caacattgaa gatggaagcg
      661 ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac
      721 cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag
      781 accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac
      841 tatacaaata gggcgcgcca gatctgttta gcttgcctcg tccccgccgg gtcacccggc
      901 cagcgacatg gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggg
//
