LOCUS       sequence-161053-         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Useful for tagging yeast proteins with C-terminal mCherry tag; 
            hygromycin-B selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Hailey DW, Davis TN, Muller EG
  TITLE     Fluorescence resonance energy transfer using color variants of green
            fluorescent protein.
  JOURNAL   Methods Enzymol. 2002;351:34-49.
  PUBMED    12073355
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2002"; volume: "351"; pages: "34-49"
COMMENT     Sequence Label: pBS35
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        56..74
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     56..73
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             135..845
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(280..298)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     548..567
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tgtcgttaga acgcggctac aattaataca taaccttatg tatcatacac atacgattta
       61 ggtgacacta tagaacgcgg ccgccagctg aagcttcgta cgctgcaggt cgacggatcc
      121 ccgggttaat taacatggtg agcaagggcg aggaggataa catggccatc atcaaggagt
      181 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
      241 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
      301 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
      361 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
      421 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
      481 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
      541 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
      601 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
      661 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
      721 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
      781 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
      841 agtagggcgc gccagatctg tttagcttgc ctcgtccccg ccgggtcacc cggccagcga
      901 catggaggcc ca
//
