LOCUS       sequence-161055-         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Useful for tagging yeast proteins with N-terminal YFP tag; G418 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Hailey DW, Davis TN, Muller EG
  TITLE     Fluorescence resonance energy transfer using color variants of green
            fluorescent protein.
  JOURNAL   Methods Enzymol. 2002;351:34-49.
  PUBMED    12073355
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2002"; volume: "351"; pages: "34-49"
COMMENT     Sequence Label: pDH22
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..725)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLKCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     protein_bind    732..765
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 taggccacta gtttgtacaa ttcatccata ccatgggtaa taccagcagc agtaacaaat
       61 tctaacaaga ccatgtggtc tctcttttcg tttggatctt tggataaggc agattgatag
      121 gataagtaat ggttgtctgg taacaagact ggaccatcac caattggagt attttgttga
      181 taatggtcag ctaattgaac agaaccatct tcaatgttgt gtctaatttt gaagttaact
      241 ttgataccat tcttttgttt gtcagccatg atgtaaacat tgtgagagtt atagttgtat
      301 tccaatttgt gacctaaaat gttaccatct tctttaaaat caataccttt taattcgatt
      361 ctattaacta aggtatcacc ttcaaacttg acttcagctc tggtcttgta gttaccgtca
      421 tctttgaaaa aaatagttct ttcttgaaca taaccttctg gcatggcaga cttgaaaaag
      481 tcatgttgtt tcatatgatc tgggtatcta gcaaaacatt ttagaccata accgaaagta
      541 gtgactaagg ttggccatgg aactggcaat ttaccagtag tacaaataaa ttttaaggtc
      601 aatttaccgt aagtagcatc accttcacct tcaccggaga cagaaaattt gtgaccatta
      661 acatcaccat ctaattcaac caaaattggg acaacaccag tgaataattc ttcaccttta
      721 gacatgatct gataacttcg tataatgtat gctatacgaa gttatctcga gagctcgttt
      781 tcgacactgg atggcggcgt tagtatcgaa tcgacagcag tatagcgacc agcattcaca
      841 tacgattgac gcatgatatt actttctgcg cacttaactt cgcatctggg cagatgatgt
      901 cgaggcgaaa a
//
