LOCUS       sequence-161056-        1033 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Useful for tagging yeast proteins with N-terminal YFP tag; G418 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Hailey DW, Davis TN, Muller EG
  TITLE     Fluorescence resonance energy transfer using color variants of green
            fluorescent protein.
  JOURNAL   Methods Enzymol. 2002;351:34-49.
  PUBMED    12073355
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2002"; volume: "351"; pages: "34-49"
COMMENT     Sequence Label: pDH22
FEATURES             Location/Qualifiers
     source          1..1033
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(89..122)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             129..842
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLKCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(877..896)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(878..896)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(983..1000)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgcgtcaatc gtatgtgaat gctggtcgct atactgctgt cgattcgata ctaacgccgc
       61 catccagtgt cgaaaacgag ctctcgagat aacttcgtat agcatacatt atacgaagtt
      121 atcagatcat gtctaaaggt gaagaattat tcactggtgt tgtcccaatt ttggttgaat
      181 tagatggtga tgttaatggt cacaaatttt ctgtctccgg tgaaggtgaa ggtgatgcta
      241 cttacggtaa attgacctta aaatttattt gtactactgg taaattgcca gttccatggc
      301 caaccttagt cactactttc ggttatggtc taaaatgttt tgctagatac ccagatcata
      361 tgaaacaaca tgactttttc aagtctgcca tgccagaagg ttatgttcaa gaaagaacta
      421 tttttttcaa agatgacggt aactacaaga ccagagctga agtcaagttt gaaggtgata
      481 ccttagttaa tagaatcgaa ttaaaaggta ttgattttaa agaagatggt aacattttag
      541 gtcacaaatt ggaatacaac tataactctc acaatgttta catcatggct gacaaacaaa
      601 agaatggtat caaagttaac ttcaaaatta gacacaacat tgaagatggt tctgttcaat
      661 tagctgacca ttatcaacaa aatactccaa ttggtgatgg tccagtcttg ttaccagaca
      721 accattactt atcctatcaa tctgccttat ccaaagatcc aaacgaaaag agagaccaca
      781 tggtcttgtt agaatttgtt actgctgctg gtattaccca tggtatggat gaattgtaca
      841 aactagtggc ctatgcggcc gcggatctgc cggtctccct atagtgagtc gtattaattt
      901 cgataagcca ggttaacctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
      961 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     1021 ggcgagcggt atc
//
