LOCUS       sequence-161061-         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Useful for tagging yeast proteins with N-terminal CFP tag; G418 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Hailey DW, Davis TN, Muller EG
  TITLE     Fluorescence resonance energy transfer using color variants of green
            fluorescent protein.
  JOURNAL   Methods Enzymol. 2002;351:34-49.
  PUBMED    12073355
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2002"; volume: "351"; pages: "34-49"
COMMENT     Sequence Label: pBS5
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..720)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKA
                     HFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     protein_bind    727..760
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cactagtttg tacaattcat ccataccatg ggtaatacca gcagcagtaa caaattctaa
       61 caagaccatg tggtctctct tttcgtttgg atctttggat aaggcagatt gagtggataa
      121 gtaatggttg tctggtaaca agactggacc atcaccaatt ggagtatttt gttgataatg
      181 gtcagctaat tgaacagaac catcttcaat gttgtgtcta attttgaaat gagctttgat
      241 accattcttt tgtttgtcag cagtgatgta aacattgtga gaaatatagt tgtattccaa
      301 tttgtgacct aaaatgttac catcttcttt aaaatcaata ccttttaatt cgattctatt
      361 aactaaggta tcaccttcaa acttgacttc agctctggtc ttgtagttac cgtcatcttt
      421 gaaaaaaata gttctttctt gaacataacc ttctggcatg gcagacttga aaaagtcatg
      481 ttgtttcata tgatctgggt atctagcaaa acattgaaca ccccaagtca aagtagtgac
      541 taaggttggc catggaactg gcaatttacc agtagtacaa ataaatttta aggtcaattt
      601 accgtaagta gcatcacctt caccttcacc ggagacagaa aatttgtgac cattaacatc
      661 accatctaat tcaaccaaaa ttgggacaac accagtgaat aattcttcac ctttagacat
      721 gatctgataa cttcgtataa tgtatgctat acgaagttat ctcgagagct cgttttcgac
      781 actggatggc ggcgttagta tcgaatcgac agcagtatag cgaccagcat tcacatacga
      841 ttgacgcatg atattacttt ctgcgcactt aacttcgcat ctgggcagat gatgtcgagg
      901 cgaaaaaaaa tataaatcac gctaacattt gattaaaata gaac
//
