LOCUS       sequence-161062-        1032 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Useful for tagging yeast proteins with N-terminal CFP tag; G418 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Hailey DW, Davis TN, Muller EG
  TITLE     Fluorescence resonance energy transfer using color variants of green
            fluorescent protein.
  JOURNAL   Methods Enzymol. 2002;351:34-49.
  PUBMED    12073355
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2002"; volume: "351"; pages: "34-49"
COMMENT     Sequence Label: pBS5
FEATURES             Location/Qualifiers
     source          1..1032
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(84..117)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             124..837
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKA
                     HFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(872..891)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(873..891)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(978..995)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caatcgtatg tgaatgctgg tcgctatact gctgtcgatt cgatactaac gccgccatcc
       61 agtgtcgaaa acgagctctc gagataactt cgtatagcat acattatacg aagttatcag
      121 atcatgtcta aaggtgaaga attattcact ggtgttgtcc caattttggt tgaattagat
      181 ggtgatgtta atggtcacaa attttctgtc tccggtgaag gtgaaggtga tgctacttac
      241 ggtaaattga ccttaaaatt tatttgtact actggtaaat tgccagttcc atggccaacc
      301 ttagtcacta ctttgacttg gggtgttcaa tgttttgcta gatacccaga tcatatgaaa
      361 caacatgact ttttcaagtc tgccatgcca gaaggttatg ttcaagaaag aactattttt
      421 ttcaaagatg acggtaacta caagaccaga gctgaagtca agtttgaagg tgatacctta
      481 gttaatagaa tcgaattaaa aggtattgat tttaaagaag atggtaacat tttaggtcac
      541 aaattggaat acaactatat ttctcacaat gtttacatca ctgctgacaa acaaaagaat
      601 ggtatcaaag ctcatttcaa aattagacac aacattgaag atggttctgt tcaattagct
      661 gaccattatc aacaaaatac tccaattggt gatggtccag tcttgttacc agacaaccat
      721 tacttatcca ctcaatctgc cttatccaaa gatccaaacg aaaagagaga ccacatggtc
      781 ttgttagaat ttgttactgc tgctggtatt acccatggta tggatgaatt gtacaaacta
      841 gtggcctatg cggccgcgga tctgccggtc tccctatagt gagtcgtatt aatttcgata
      901 agccaggtta acctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
      961 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     1021 gcggtatcag ct
//
