LOCUS       sequence-161063-         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Useful for tagging yeast proteins with N-terminal Venus tag; G418 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Hailey DW, Davis TN, Muller EG
  TITLE     Fluorescence resonance energy transfer using color variants of green
            fluorescent protein.
  JOURNAL   Methods Enzymol. 2002;351:34-49.
  PUBMED    12073355
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2002"; volume: "351"; pages: "34-49"
COMMENT     Sequence Label: pBES2
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    728..761
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ccactagttt gtacaattca tccataccat gggtaatacc agcagcagta acaaattcta
       61 acaagaccat gtggtctctc ttttcgtttg gatctttgga taaggcagat tgataggata
      121 agtaatggtt gtctggtaac aagactggac catcaccaat tggagtattt tgttgataat
      181 ggtctgctag ttgaacacct ccatcttcaa tgttgtgtct aattttgaag ttagctttga
      241 ttccattctt ttgtttgtct gcagtgatgt atacattgtg tgagttatag ttgtattcca
      301 atttgtgtcc aagaatgttt ccatcttctt taaaatcaat accttttaac tcgattctat
      361 taacaagggt atcaccttca aacttgactt cagcacgtgt cttgtagttc ccgtcatctt
      421 tgaaaaatat agttctttcc tgtacataac cttcgggcat ggcactcttg aaaaagtcat
      481 gccgtttcat atgatctggg tatctagcaa agcacattag accataaccc aaagtagtga
      541 caagtgttgg ccatggaact ggcaatttac cagtagtaca aatcaatttt aaggtcaatt
      601 taccgtaagt agcatcacct tcaccttcac cggagacaga aaatttgtga ccattaacat
      661 caccatctaa ttcaaccaaa attgggacaa caccagtgaa taattcttca cctttagaca
      721 tgatctgata acttcgtata atgtatgcta tacgaagtta tctcgagagc tcgttttcga
      781 cactggatgg cggcgttagt atcgaatcga cagcagtata gcgaccagca ttcacatacg
      841 attgacgcat gatattactt tctgcgcact taacttcgca tctgggcaga tgatgtcgag
      901 gcgaaaaaa
//
