LOCUS       sequence-161066-         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Useful for tagging yeast proteins with N-terminal Venus tag; G418 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Hailey DW, Davis TN, Muller EG
  TITLE     Fluorescence resonance energy transfer using color variants of green
            fluorescent protein.
  JOURNAL   Methods Enzymol. 2002;351:34-49.
  PUBMED    12073355
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2002"; volume: "351"; pages: "34-49"
COMMENT     Sequence Label: pBES2
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(75..108)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(863..882)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(864..882)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gtgaatgctg gtcgctatac tgctgtcgat tcgatactaa cgccgccatc cagtgtcgaa
       61 aacgagctct cgagataact tcgtatagca tacattatac gaagttatca gatcatgtct
      121 aaaggtgaag aattattcac tggtgttgtc ccaattttgg ttgaattaga tggtgatgtt
      181 aatggtcaca aattttctgt ctccggtgaa ggtgaaggtg atgctactta cggtaaattg
      241 accttaaaat tgatttgtac tactggtaaa ttgccagttc catggccaac acttgtcact
      301 actttgggtt atggtctaat gtgctttgct agatacccag atcatatgaa acggcatgac
      361 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat
      421 gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga
      481 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa
      541 tacaactata actcacacaa tgtatacatc actgcagaca aacaaaagaa tggaatcaaa
      601 gctaacttca aaattagaca caacattgaa gatggaggtg ttcaactagc agaccattat
      661 caacaaaata ctccaattgg tgatggtcca gtcttgttac cagacaacca ttacttatcc
      721 tatcaatctg ccttatccaa agatccaaac gaaaagagag accacatggt cttgttagaa
      781 tttgttactg ctgctggtat tacccatggt atggatgaat tgtacaaact agtggcctat
      841 gcggccgcgg atctgccggt ctccctatag tgagtcgtat taatttcgat aagccaggtt
      901 aacctgcatt aatgaatcgg ccaa
//
