LOCUS       sequence-161073-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for N-terminal fusion with 
            FLAG (CaMV35S promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB512
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(86..92,93..110,111..116))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(141..265)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(275..298)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(319..664)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(356..376)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
       61 gacggatcct aactcaaaat ccacacatta tacgagccgg aagcataaag tgtaaagcct
      121 ggggtgccta atgcggccgc catagtgact ggatatgttg tgttttacag tattatgtag
      181 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
      241 gttcagcttt tttgtacaaa cttgtgataa cagccttgtc atcgtcatcc ttgtagtcgc
      301 tcattctaga gtcccccgtg ttctctccaa atgaaatgaa cttccttata tagaggaagg
      361 gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca
      421 atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg
      481 tgggggtcca tctttgggac cactgtcggc agaggcatct tcaacgatgg cctttccttt
      541 atcgcaatga tggcatttgt aggagccacc ttccttttcc actatcttca caataaagtg
      601 acagatagct gggcaatgga atccgaggag gtttccggat attacccttt gttgaaaagt
      661 ctcaattgcc ctttggtctt ctgagactgt atctttgata tttttggagt agacaagtgt
      721 gtcgtgctcc accatgttga cgaagatttt cttcttgtca ttgagtcgta agagactctg
      781 tatgaactgt tcgccagtct ttacggcgag ttctgttagg tcctctattt gaatctttga
      841 ctccatggcc tttgattcag tgggaactac ctttttagag actccaatct ctattacttg
//
