LOCUS       sequence-161074-         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  YFP-CFP positive FRET control; his selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Hailey DW, Davis TN, Muller EG
  TITLE     Fluorescence resonance energy transfer using color variants of green
            fluorescent protein.
  JOURNAL   Methods Enzymol. 2002;351:34-49.
  PUBMED    12073355
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2002"; volume: "351"; pages: "34-49"
COMMENT     Sequence Label: pDH18
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(50..760)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAH
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     705..733
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 aaaatcataa atcataagaa attcgcttat ttagaagtgg cgcgccctat ttgtatagtt
       61 catccatgcc atgtgtaatc ccagcagctg ttacaaactc aagaaggacc atgtggtctc
      121 tcttttcgtt gggatctttc gaaagggcag attgtgtgga caggtaatgg ttgtctggta
      181 aaaggacagg gccatcgcca attggagtat tttgttgata atggtctgct agttgaacgc
      241 ttccatcttc aatgttgtgt ctaattttga aatgagcttt gattccattc ttttgtttgt
      301 ctgcagtgat gtatacattg tgtgaaatat agttgtattc caatttgtgt ccaagaatgt
      361 ttccatcttc tttaaaatca atacctttta actcgattct attaacaagg gtatcacctt
      421 caaacttgac ttcagcacgt gtcttgtagt tcccgtcatc tttgaaaaat atagttcttt
      481 cctgtacata accttcgggc atggcactct tgaaaaagtc atgccgtttc atatgatctg
      541 ggtatcttga aaagcattga acaccccaag tcaaagtagt gacaagtgtt ggccatggaa
      601 caggtagttt tccagtagtg caaataaatt taagggtaag ttttccgtat gttgcatcac
      661 cttcaccctc tccactgaca gaaaatttgt gcccattaac atcaccatct aattcaacaa
      721 gaattgggac aactccagtg aaaagttctt ctcctttact ggcgcctttg tatagttcat
      781 ccatgccatg tgtaatccca gcagctgtta caaactcaag aaggaccatg tggtctctct
      841 tttcgttggg atctttcgaa agggcagatt gataggacag gtaatggttg tctggtaaaa
      901 ggacagggcc atcgccaatt ggagtatttt gttgataatg gtctgctagt tgaacgcttc
      961 catc
//
