LOCUS       sequence-161076-         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pBIG1a - Step1 vector a (biGBac system for expression of protein 
            complexes in insect cells).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Weissmann F, Petzold G, VanderLinden R, Huis In 't Veld PJ, Brown 
            NG, Lampert F, Westermann S, Stark H, Schulman BA, Peters JM
  TITLE     biGBac enables rapid gene assembly for the expression of large 
            multisubunit protein complexes.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2564-9. doi: 
            10.1073/pnas.1604935113. Epub 2016 Apr 25.
  PUBMED    27114506
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1604935113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-05-10- 10"; volume: "113"; issue: "19"; pages: "E2564-9"
COMMENT     Sequence Label: pBIG1a
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(7..40)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(86..105)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    130..178
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     mobile_element  455..620
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ggtaccataa cttcgtatag catacattat acgaagttat ctgggtttaa acgctctatg
       61 gtctaaagtt tttatcgagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc
      121 gcgctatgac ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcat
      181 aaacgcgggg ttcggtccca gggctggcac tctgtcaagt gaacgctcta tggtctaaag
      241 atttaaatct atatctcaat cggggttacg tcgatacccc accgagaccc cattgggacc
      301 aatacgcccg cgtttcttcc ttttccccac cccaaccccc aagttcgggt gaaggcccag
      361 ggctcgcagc caacgtcggg gcggcaagcc ctgccatagc cactacgggt taattaaaaa
      421 ctatatctca atcggggttt aaacaggaga tccgaaccag ataagtgaaa tctagttcca
      481 aactattttg tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct
      541 attttgtcac tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc
      601 aactattttg tccgcccaca gcggggcatt tttcttcctg ttatgttttt aatcaaacat
      661 cctgccaact ccatgtgaca aaccgtcatc ttcggctact ttttctctgt cacagaatga
      721 aaatttttct gtcatctctt cgttattaat gtttgtaatt gactgaatat caacgcttat
      781 ttgcagcctg aactgggagg cagaataaat gatcatatcg tcaattatta cctccacggg
      841 gagagcctga gcaaactggc ctcaggcatt tgagaagcac acggtcacac tgcttccggt
      901 agtcaataaa ccggtaagta gcgta
//
