LOCUS       sequence-161090-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Nuf2 N-terminally labeled with mTurquoise2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Yoo TY, Choi JM, Conway W, Yu CH, Pappu RV, Needleman DJ
  TITLE     Measuring NDC80 binding reveals the molecular basis of 
            tension-dependent kinetochore-microtubule attachments.
  JOURNAL   Elife. 2018 Jul 25;7. pii: 36392. doi: 10.7554/eLife.36392.
  PUBMED    30044223
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-07-25"; volume: "7. pii: 36392. doi"; pages: " 
            10.7554/eLife.36392"
COMMENT     Sequence Label: pJAG98-mTurquoise2-Nuf2
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             51..449
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
ORIGIN
        1 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttc atggccaagc
       61 ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc
      121 ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca
      181 ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca
      241 ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca
      301 ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga
      361 tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc
      421 tgccctctgg ttatgtgtgg gagggctaag atcgataaaa taaaagattt tatttagtct
      481 ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac
      541 gccattttgc aaggcatgga aaaatacata actgagaata gagaagttca gatcaaggtc
      601 aggaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
      661 ccccggctca gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt
      721 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag
      781 ccctcagcag tttctagaga accatcagat gtttccaggg tgcc
//
