LOCUS       sequence-161097-         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector targeting the eGFP gene to the GAPDH locus of human cells. 
            eGFP is expressed from a 2A sequence at the C terminus of GAPDH. 
            G418 resistance is encoded by the Meo gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris
            FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E,
            Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ,
            Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG
  TITLE     GAPTrap: A Simple Expression System for Pluripotent Stem Cells and 
            Their Derivatives.
  JOURNAL   Stem Cell Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015.
  PUBMED    27594589
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015."
COMMENT     Sequence Label: pGAPTrap-eGFP-IRESMeo
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     215..236
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(288..341)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag
       61 cggctgaagc actgcacgcc gtaggtcagg gtggtcacga gggtgggcca gggcacgggc
      121 agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg
      181 ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg
      241 ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tacgcgccgg gccaggattc
      301 tcctccacgt caccgcatgt tagaagactt cctctgccct ctcctccgga cccgccgcca
      361 cgcgccattc cctccttgga ggccatgtgg gccatgaggt ccaccaccct gttgctgtag
      421 ccaaattcgt tgtcatacct agaagatgaa aagagttgtc agggcccttt ttctgagcca
      481 gccaccagag ggcgccagac cctgcacttt ttaagagcca gtctctggcc ccagccacat
      541 accaggaaat gagcttgaca aagtggtcgt tgagggcaat gccagcccca gcgtcaaagg
      601 tggaggagtg ggtgtcgctg ttgaagtcag aggagaccac ctggtgctca gtgtagccca
      661 ggatgccctt gagggggccc tccgacgcct gcttcaccac cttcttgatg tcatcatatt
      721 tggcaggttt ttctagacgg caggtcaggt ccaccactga cacgttggca gtggggacac
      781 ggaaggccat gccagtgagc ttcccgttca gctca
//
