LOCUS       sequence-161108-         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector targeting the eGFP gene to the GAPDH locus of human cells. 
            eGFP is expressed from a 2A sequence at the C terminus of GAPDH. 
            Puromycin resistance is encoded by the Muro gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris
            FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E,
            Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ,
            Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG
  TITLE     GAPTrap: A Simple Expression System for Pluripotent Stem Cells and 
            Their Derivatives.
  JOURNAL   Stem Cell Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015.
  PUBMED    27594589
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015."
COMMENT     Sequence Label: pGAPTrap-eGFP-IRESMuro
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     211..232
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(284..337)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc
       61 tgaagcactg cacgccgtag gtcagggtgg tcacgagggt gggccagggc acgggcagct
      121 tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct
      181 cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca
      241 ccaccccggt gaacagctcc tcgcccttgc tcaccatacg cgccgggcca ggattctcct
      301 ccacgtcacc gcatgttaga agacttcctc tgccctctcc tccggacccg ccgccacgcg
      361 ccattccctc cttggaggcc atgtgggcca tgaggtccac caccctgttg ctgtagccaa
      421 attcgttgtc atacctagaa gatgaaaaga gttgtcaggg ccctttttct gagccagcca
      481 ccagagggcg ccagaccctg cactttttaa gagccagtct ctggccccag ccacatacca
      541 ggaaatgagc ttgacaaagt ggtcgttgag ggcaatgcca gccccagcgt caaaggtgga
      601 ggagtgggtg tcgctgttga agtcagagga gaccacctgg tgctcagtgt agcccaggat
      661 gcccttgagg gggccctccg acgcctgctt caccaccttc ttgatgtcat catatttggc
      721 aggtttttct agacggcagg tcaggtccac cactgacacg ttggcagtgg ggacacggaa
      781 ggccatgcca gtgagcttcc cgttcagctc agggatgacc ttgcccacag ccttggcagc
      841 gccagtagag gcagggatga tgttctggag agccccgcgg ccatcacgcc acagtttccc
      901 ggaggggcca tccacagtct tctgggtggc agtgatggca tg
//
