LOCUS       sequence-161112-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector targeting the eGFP gene to the GAPDH locus of human cells. 
            eGFP is expressed from a 2A sequence at the C terminus of GAPDH. 
            Puromycin resistance is encoded by the Muro gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris
            FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E,
            Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ,
            Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG
  TITLE     GAPTrap: A Simple Expression System for Pluripotent Stem Cells and 
            Their Derivatives.
  JOURNAL   Stem Cell Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015.
  PUBMED    27594589
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015."
COMMENT     Sequence Label: pGAPTrap-eGFP-IRESMuro
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cccattgtat gggatctgat ctggggcctc ggtacacatg ctttacatgt gtttagtcga
       61 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
      121 gatgataata tggccacaac catggcgcgt atggcaagac tgaccgaata caagcctacc
      181 gtgagactgg caacaagaga tgatgtccca agagccgtgc gaaccctggc agcagcattc
      241 gcagactacc cagccacaag acacactgtg gaccccgata ggcatatcga gagagtgacc
      301 gagctgcagg aactgtttct gacacgggtg ggcctggata ttggaaaggt ctgggtggca
      361 gatgatgggg ctgctgtggc cgtctggaca accccagagt ctgtggaagc tggagctgtc
      421 ttcgctgaga tcggccctcg aatggcagaa ctgtctggct ccaggctggc agcacagcag
      481 cagatggagg gactgctggc accacacaga cctaaggaac cagcttggtt cctggcaact
      541 gtgggggtct ctcccgacca tcagggcaaa ggactgggga gtgccgtggt cctgcctgga
      601 gtggaggctg cagaaagagc tggagtccca gcttttctgg agacatctgc cccccggaac
      661 ctgcctttct atgaacgcct gggctttact gtgaccgctg acgtggaggt gcccgaaggc
      721 ccccgaactt ggtgcatgac ccgaaagcca ggagcataaa tctagacgcg tatcgataag
      781 cttagatcga gcacaagagg aagagagaga ccctcactgc tggggagtcc ctgccacact
      841 cagtccc
//
