LOCUS       sequence-161115-         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector targeting the mCherry gene to the GAPDH locus of human cells.
            mCherry is expressed from a 2A sequence at the C terminus of GAPDH. 
            Hygromycin B resistance is encoded by the Mygro gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris
            FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E,
            Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ,
            Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG
  TITLE     GAPTrap: A Simple Expression System for Pluripotent Stem Cells and 
            Their Derivatives.
  JOURNAL   Stem Cell Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015.
  PUBMED    27594589
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015."
COMMENT     Sequence Label: pGAPTrap-mCherry-IRESMygro
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tacacatgct ttacatgtgt
       61 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
      121 aaaaacacga tgataatatg gccacaacca tggcgcgtat gaaaaagccc gagctgactg
      181 ccacctctgt cgagaagttc ctgattgaaa agtttgattc cgtgagtgac ctgatgcagc
      241 tgagcgaggg cgaggaatcc cgggccttct cttttgacgt gggcggaagg ggctacgtgc
      301 tgagagtcaa ctcttgcgcc gacggattct ataaggatcg ctacgtgtat cgacactttg
      361 ctagtgccgc tctgcccatc cctgaggtgc tggacattgg cgagttcagc gaatccctga
      421 catactgtat ctccaggaga gcccagggag tgactctgca ggatctgcct gagaccgaac
      481 tgcctgccgt gctgcagcct gtggctgaag ctatggacgc tatcgcagcc gctgatctgt
      541 cacagactag cgggttcggc ccatttggcc cccagggaat tgggcagtac accacatgga
      601 gagactttat ctgcgcaatt gccgatcccc acgtgtatca ttggcagaca gtcatggacg
      661 ataccgtgag cgccagtgtc gctcaggcac tggacgagct gatgctgtgg gcagaggatt
      721 gtcctgaagt gaggcacctg gtccatgctg acttcgggag caacaatgtg ctgaccgata
      781 acggcagaat cacagccgtc attgactgga gtgaggctat gtttggcgat tcacagtacg
      841 aagtggctaa tatcttcttt tggcgaccct gg
//
