LOCUS       sequence-161117-         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector targeting the mCherry gene to the GAPDH locus of human cells.
            mCherry is expressed from a 2A sequence at the C terminus of GAPDH. 
            Hygromycin B resistance is encoded by the Mygro gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris
            FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E,
            Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ,
            Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG
  TITLE     GAPTrap: A Simple Expression System for Pluripotent Stem Cells and 
            Their Derivatives.
  JOURNAL   Stem Cell Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015.
  PUBMED    27594589
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015."
COMMENT     Sequence Label: pGAPTrap-mCherry-IRESMygro
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(113..166)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 cctcgatctc gaactcgtgg ccgttcacgg agccctccat gtgcaccttg aagcgcatga
       61 actccttgat gatggccatg ttatcctcct cgcccttgct caccatacgc gccgggccag
      121 gattctcctc cacgtcaccg catgttagaa gacttcctct gccctctcct ccggacccgc
      181 cgccacgcgc cattccctcc ttggaggcca tgtgggccat gaggtccacc accctgttgc
      241 tgtagccaaa ttcgttgtca tacctagaag atgaaaagag ttgtcagggc cctttttctg
      301 agccagccac cagagggcgc cagaccctgc actttttaag agccagtctc tggccccagc
      361 cacataccag gaaatgagct tgacaaagtg gtcgttgagg gcaatgccag ccccagcgtc
      421 aaaggtggag gagtgggtgt cgctgttgaa gtcagaggag accacctggt gctcagtgta
      481 gcccaggatg cccttgaggg ggccctccga cgcctgcttc accaccttct tgatgtcatc
      541 atatttggca ggtttttcta gacggcaggt caggtccacc actgacacgt tggcagtggg
      601 gacacggaag gccatgccag tgagcttccc gttcagctca gggatgacct tgcccacagc
      661 cttggcagcg ccagtagagg cagggatgat gttctggaga gccccgcggc catcacgcca
      721 cagttt
//
