LOCUS       sequence-161118-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector targeting the eGFP gene to the GAPDH locus of human cells. 
            eGFP is expressed from a 2A sequence at the C terminus of GAPDH..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris
            FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E,
            Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ,
            Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG
  TITLE     GAPTrap: A Simple Expression System for Pluripotent Stem Cells and 
            Their Derivatives.
  JOURNAL   Stem Cell Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015.
  PUBMED    27594589
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015."
COMMENT     Sequence Label: pGAPTrap-GFP
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     151..172
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
       61 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
      121 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
      181 cgggatcact ctcggcatgg acgagctgta caagtaagga tccactgagt tatcgataag
      241 cttagatcga gcacaagagg aagagagaga ccctcactgc tggggagtcc ctgccacact
      301 cagtccccca ccacactgaa tctcccctcc tcacagttgc catgtagacc ccttgaagag
      361 gggaggggcc tagggagccg caccttgtca tgtaccatca ataaagtacc ctgtgctcaa
      421 ccagttactt gtcctgtctt attctagggt ctggggcaga ggggagggaa gctgggcttg
      481 tgtcaaggtg agacattctt gctggggagg gacctggtat gttctcctca gactgagggt
      541 agggcctcca aacagccttg cttgcttcga gaaccatttg cttcccgctc agacgtcttg
      601 agtgctacag gaagctggca ccactacttc agagaacaag gccttttcct ctcctcgctc
      661 cagtcctagg ctatctgctg ttggccaaac atggaagaag ctattctgtg ggcagcccca
      721 gggaggctga caggtggagg aagtcagggc tcgcactggg ctctgacgct gactggttag
      781 tggagctcag cctggagctg agctgcagcg ggcaattcca gcttggcctc cgcagctgt
//
