LOCUS       sequence-161120-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector targeting the eGFP gene to the GAPDH locus of human cells. 
            eGFP is expressed from a 2A sequence at the C terminus of GAPDH..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris
            FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E,
            Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ,
            Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG
  TITLE     GAPTrap: A Simple Expression System for Pluripotent Stem Cells and 
            Their Derivatives.
  JOURNAL   Stem Cell Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015.
  PUBMED    27594589
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015."
COMMENT     Sequence Label: pGAPTrap-GFP
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     217..238
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(290..343)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt
       61 agcggctgaa gcactgcacg ccgtaggtca gggtggtcac gagggtgggc cagggcacgg
      121 gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct
      181 cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga
      241 tgggcaccac cccggtgaac agctcctcgc ccttgctcac catacgcgcc gggccaggat
      301 tctcctccac gtcaccgcat gttagaagac ttcctctgcc ctctcctccg gacccgccgc
      361 cacgcgccat tccctccttg gaggccatgt gggccatgag gtccaccacc ctgttgctgt
      421 agccaaattc gttgtcatac ctagaagatg aaaagagttg tcagggccct ttttctgagc
      481 cagccaccag agggcgccag accctgcact ttttaagagc cagtctctgg ccccagccac
      541 ataccaggaa atgagcttga caaagtggtc gttgagggca atgccagccc cagcgtcaaa
      601 ggtggaggag tgggtgtcgc tgttgaagtc agaggagacc acctggtgct cagtgtagcc
      661 caggatgccc ttgagggggc cctccgacgc ctgcttcacc accttcttga tgtcatcata
      721 tttggcaggt ttttctagac ggcaggtcag gtccaccact gacacgttgg cagtggggac
      781 acggaaggcc atgccagtga gcttcccgtt cagctcagg
//
