LOCUS       sequence-161121-         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The catalytically inactive genetically encoded fluorescent H2O2 
            sensor roGFP2-Tsa2-Delta-CP-Delta-CR cloned in the yeast p415 vector
            under the control of a TEF promoter. For cytosolic expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Morgan B, Van Laer K, Owusu TN, Ezerina D, Pastor-Flores D, Amponsah
            PS, Tursch A, Dick TP
  TITLE     Real-time monitoring of basal H2O2 levels with peroxiredoxin-based 
            probes.
  JOURNAL   Nat Chem Biol. 2016 Jun;12(6):437-43. doi: 10.1038/nchembio.2067. 
            Epub 2016 Apr 18.
  PUBMED    27089028
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2067"; journalName: "Nat Chem Biol"; date: 
            "2016-06"; volume: "12"; issue: "6"; pages: "437-43"
COMMENT     Sequence Label: p415 TEF roGFP2‐Tsa2-Delta-CP-Delta-CR
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      409..656
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(425..443)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(674..693)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(675..693)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(703..720)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(703..719)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gatcagggcg cccaagtttt atttgcctcc accgactctg aatattcctt actggcatgg
       61 accaaccttc ccagaaaaga cggtggatta ggtccagtta aagttccttt gcttgctgat
      121 aagaatcatt ccttatccag agactatggc gttttgattg aaaaagaagg tatagcttta
      181 agaggtttgt tcataatcga cccgaaggga atcattagac atatcactat caatgattta
      241 tctgttggca gaaacgtcaa tgaagctttg agattagtcg aaggtttcca gtggactgac
      301 aaaaatggta cagttttgcc agccaactgg accccaggag ccgccaccat caaacctgac
      361 gttaaagatt ccaaggagta tttcaaaaat gccaataatt aactcgagtc atgtaattag
      421 ttatgtcacg cttacattca cgccctcccc ccacatccgc tctaaccgaa aaggaaggag
      481 ttagacaacc tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa
      541 cgttatttat atttcaaatt tttctttttt ttctgtacag acgcgtgtac gcatgtaaca
      601 ttatactgaa aaccttgctt gagaaggttt tgggacgctc gaaggcttta atttgcggcc
      661 ggtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
      721 acgtcgtgac tgg
//
