LOCUS       sequence-161124-         627 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2-Delta-CR
            cloned in the yeast p415 vector under the control of a TEF promoter.
            For mitochondrial matrix expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Morgan B, Van Laer K, Owusu TN, Ezerina D, Pastor-Flores D, Amponsah
            PS, Tursch A, Dick TP
  TITLE     Real-time monitoring of basal H2O2 levels with peroxiredoxin-based 
            probes.
  JOURNAL   Nat Chem Biol. 2016 Jun;12(6):437-43. doi: 10.1038/nchembio.2067. 
            Epub 2016 Apr 18.
  PUBMED    27089028
REFERENCE   2  (bases 1 to 627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2067"; journalName: "Nat Chem Biol"; date: 
            "2016-06"; volume: "12"; issue: "6"; pages: "437-43"
COMMENT     Sequence Label: p415 TEF Su9‐roGFP2‐Tsa2-Delta-CR
FEATURES             Location/Qualifiers
     source          1..627
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(401..419)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     terminator      431..620
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
ORIGIN
        1 gcctccaccg actctgaata ttccttactg gcatggacca accttcccag aaaagacggt
       61 ggattaggtc cagttaaagt tcctttgctt gctgataaga atcattcctt atccagagac
      121 tatggcgttt tgattgaaaa agaaggtata gctttaagag gtttgttcat aatcgacccg
      181 aagggaatca ttagacatat cactatcaat gatttatctg ttggcagaaa cgtcaatgaa
      241 gctttgagat tagtcgaagg tttccagtgg actgacaaaa atggtacagt tttgccagcc
      301 aactggaccc caggagccgc caccatcaaa cctgacgtta aagattccaa ggagtatttc
      361 aaaaatgcca ataattaact cgagtcatgt aattagttat gtcacgctta cattcacgcc
      421 ctccccccac atccgctcta accgaaaagg aaggagttag acaacctgaa gtctaggtcc
      481 ctatttattt ttttatagtt atgttagtat taagaacgtt atttatattt caaatttttc
      541 ttttttttct gtacagacgc gtgtacgcat gtaacattat actgaaaacc ttgcttgaga
      601 aggttttggg acgctcgaag gctttaa
//
