LOCUS       sequence-161128-         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Residues 1-359 of ALIX; Bacterial Expression Vector; N-term His-tag;
            Sundquist Lab Internal ID: WISP11-428.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  AUTHORS   McCullough J, Fisher RD, Whitby FG, Sundquist WI, Hill CP
  TITLE     ALIX-CHMP4 interactions in the human ESCRT pathway.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Jun 3;105(22):7687-91. doi: 
            10.1073/pnas.0801567105. Epub 2008 May 29.
  PUBMED    18511562
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0801567105"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2008-06-3- 3"; volume: "105"; issue: "22"; pages: "7687-91"
COMMENT     Sequence Label: pet16b ALIX Bro1 (1-359)
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(338..356)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      352..399
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(574..579,580..596,597..602))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(629..647)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aacagcagaa gaaatttgga gaagaaattg caaggttaca gcatgcagca gaactgatta
       61 aaacagtggc atctcgctat gatgaatatg ttaatgtgaa ggatttttct gacaaaatca
      121 atcgtgccct tgctgcagca aagaaggata atgacttcat ttatcatgat cgagttccag
      181 accttaaaga tctagatcct attggcaaag ccacacttgt gaaatctacc ccggtcaatg
      241 tacccatcag tcagaaattt actgatctgt ttgagaagat ggtttaagga tccggctgct
      301 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa
      361 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc
      421 ggatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca
      481 gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac
      541 tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc
      601 aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
      661 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
      721 acccctatt
//
