LOCUS       sequence-161129-         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector targeting the Firefly Luciferase (Luc2) gene to the GAPDH 
            locus of human cells. It is expressed from a 2A sequence at the C 
            terminus of GAPDH. Puromycin resistance is encoded by the Meo gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris
            FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E,
            Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ,
            Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG
  TITLE     GAPTrap: A Simple Expression System for Pluripotent Stem Cells and 
            Their Derivatives.
  JOURNAL   Stem Cell Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015.
  PUBMED    27594589
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015."
COMMENT     Sequence Label: pGAPTrap-Luc2-IRESMeo
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgtcaccgca tgttagaaga cttcctctgc cctctcctcc ggacccgccg ccacgcgcca
       61 ttccctcctt ggaggccatg tgggccatga ggtccaccac cctgttgctg tagccaaatt
      121 cgttgtcata cctagaagat gaaaagagtt gtcagggccc tttttctgag ccagccacca
      181 gagggcgcca gaccctgcac tttttaagag ccagtctctg gccccagcca cataccagga
      241 aatgagcttg acaaagtggt cgttgagggc aatgccagcc ccagcgtcaa aggtggagga
      301 gtgggtgtcg ctgttgaagt cagaggagac cacctggtgc tcagtgtagc ccaggatgcc
      361 cttgaggggg ccctccgacg cctgcttcac caccttcttg atgtcatcat atttggcagg
      421 tttttctaga cggcaggtca ggtccaccac tgacacgttg gcagtgggga cacggaaggc
      481 catgccagtg agcttcccgt tcagctcagg gatgaccttg cccacagcct tggcagcgcc
      541 agtagaggca gggatgatgt tctggagagc cccgcggcca tcacgccaca gtttcccgga
      601 ggggccatcc acagtcttct gggtggcagt gatggcatgg actgtggtct gcaaaaggag
      661 tgaggccctg cagcgtactc cccacatcac ccctctacct ccctccccac cttgaaagga
      721 aattatggga aagccagtcc ccagaacccc caaccccagg gttgcacgca gccccggcag
      781 ggaggagcca gtcttggatg agaaaggtgg gagcctcagt cccattcccc agctctcata
      841 ccatgagtcc ttccacgata ccaaagttgt catggatgac cttggccagg ggtgctaagc
      901 agttggtggt gcaggaggca ttgctgcaa
//
