LOCUS       sequence-161132-         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector targeting the Firefly Luciferase (Luc2) gene to the GAPDH 
            locus of human cells. It is expressed from a 2A sequence at the C 
            terminus of GAPDH. Puromycin resistance is encoded by the Meo gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris
            FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E,
            Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ,
            Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG
  TITLE     GAPTrap: A Simple Expression System for Pluripotent Stem Cells and 
            Their Derivatives.
  JOURNAL   Stem Cell Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015.
  PUBMED    27594589
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015."
COMMENT     Sequence Label: pGAPTrap-Luc2-IRESMeo
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ccattgtatg ggatctgatc tggggcctcg gtacacatgc tttacatgtg tttagtcgag
       61 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
      121 atgataatat ggccacaacc atggcgcgta tggctcgggg cattgaacag gatgggctgc
      181 acgccgggtc acctgctgct tgggtcgaaa gactgtttgg gtatgattgg gctcagcaga
      241 caatcggctg ctctgacgcc gccgtgttca ggctgagtgc tcagggacgc cccgtgctgt
      301 ttgtcaagac tgacctgtca ggggcactga acgagctgca ggatgaggca gcccgcctga
      361 gctggctggc aaccacagga gtgccttgtg ctgcagtcct ggacgtggtc actgaggccg
      421 gacgagattg gctgctgctg ggagaagtgc caggacagga cctgctgagc tcccacctgg
      481 caccagctga gaaggtctcc atcatggcag atgccatgag gagactgcat actctggacc
      541 ccgccacctg ccctttcgat caccaggcta aacatcgcat tgagcgggct cgcacacgaa
      601 tggaagcagg cctggtggac caggacgatc tggatgagga acaccaggga ctggctcctg
      661 cagagctgtt tgcaaggctg aaagccagaa tgccagacgg cgaagatctg gtggtcaccc
      721 atggagacgc ttgcctgccc aacatcatgg tggagaatgg aagattcagt gggtttattg
      781 attgtggccg actgggagtc gccgacaggt accaggatat cgccctggct acaagagaca
      841 ttgcagagga actgtgcggg gaatgggccg atcggttcct ggtgctgtat ggcattgctg
      901 ctcc
//
