LOCUS       sequence-161136-         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RFP selectable marker for use in two-stage CRISPR-mediated allele 
            replacement strategies in Drosophila. D. americana tan homology arms
            can be replaced with locus of choice..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Lamb AM, Walker EA, Wittkopp PJ
  TITLE     Tools and strategies for scarless allele replacement in Drosophila 
            using CRISPR/Cas9.
  JOURNAL   Fly (Austin). 2016 Aug 5:1-12.
  PUBMED    27494619
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fly 
            (Austin). 2016 Aug 5:1-12."
COMMENT     Sequence Label: pGEM-3XP3.RFP-tan
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aagctaagca aataaacaag cgcagctgaa caagctaaac aatcgggcgg ccgcactaga
       61 gccggtcgcc accatgaggt cttccaagaa tgttatcaag gagttcatga ggtttaaggt
      121 tcgcatggaa ggaacggtca atgggcacga gtttgaaata gaaggcgaag gagaggggag
      181 gccatacgaa ggccacaata ccgtaaagct taaggtaacc aaggggggac ctttgccatt
      241 tgcttgggat attttgtcac cacaatttca gtatggaagc aaggtatatg tcaagcaccc
      301 tgccgacata ccagactata aaaagctgtc atttcctgaa ggatttaaat gggaaagggt
      361 catgaacttt gaagacggtg gcgtcgttac tgtaacccag gattccagtt tgcaggatgg
      421 ctgtttcatc tacaaggtca agttcattgg cgtgaacttt ccttccgatg gacctgttat
      481 gcaaaagaag acaatgggct gggaagccag cactgagcgt ttgtatcctc gtgatggcgt
      541 gttgaaagga gagattcata aggctctgaa gctgaaagac ggtggtcatt acctagttga
      601 attcaaaagt atttacatgg caaagaagcc tgtgcagcta ccagggtact actatgttga
      661 ctccaaactg gatataacaa gccacaacga agactataca atcgttgagc agtatgaaag
      721 aaccgaggga cgccaccatc tgttccttta gcggccatcg aattcgagct cgcccactaa
      781 gcgtcgcgcc acttcaacgc tcgatgggag cgtcattggt gggcggggta accgtcgaaa
      841 tcagtgttta cgcttccaat cgcaacaaaa aattcactgc aacactgaaa agcatacgaa
      901 aacgatgaag attgtacgag aaaccataaa gtattttatc c
//
