LOCUS       sequence-161139-        1081 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RFP selectable marker for use in two-stage CRISPR-mediated allele 
            replacement strategies in Drosophila. D. americana tan homology arms
            can be replaced with locus of choice..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Lamb AM, Walker EA, Wittkopp PJ
  TITLE     Tools and strategies for scarless allele replacement in Drosophila 
            using CRISPR/Cas9.
  JOURNAL   Fly (Austin). 2016 Aug 5:1-12.
  PUBMED    27494619
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fly 
            (Austin). 2016 Aug 5:1-12."
COMMENT     Sequence Label: pGEM-3XP3.RFP-tan
FEATURES             Location/Qualifiers
     source          1..1081
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(148..181)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 agnctcgnan ngagcgcctc tatttatact ccggcggtcg agggttcgaa atcgataagc
       61 ttggatccta attgaattag ctctaattga attagtctct aattgaatta gatccccggg
      121 cgagctcgcg gccgcgaatt cgtcgacata acttcgtata gcatacatta tacgaagtta
      181 tgagctcaat tggtaccgta cgagatcttg ccggaggaca tgctggacga catttggtgg
      241 gccaccgggg aggtgcacat ggcttcctgg gctttcgttt tggtctatgt gctgtggacc
      301 gtttatcgct cttcgcgcta tttggctctg aaattcgagc aggatgagca gtaataatat
      361 gctctcaatt attcttttct tttctttttt ttttttttgc ttgggttcgt cttatccacg
      421 atcacaaaat gtaaatagaa agacgaaaac aaaaataaag cacaggaaat atatataata
      481 attcagtggg aataaaaact cgctggaaaa gtgaattaca acaaattttg caacaaaaac
      541 aaaacaaaat tcacaagatg aaataataat aataataaaa aaaaacacaa ctgagttaat
      601 aatttcatta aacaaacttt accaacactc aaacacacgc acgcacacac agagacagag
      661 cgagagaaag gttacgagag agagagagaa tttcagtgta aacaaattgt atatagataa
      721 ttcaagccaa gtttttatat gattgttcta aagcgcttga aggcagaatt caaactgaat
      781 tggacatgtt ttggcgggca gcaattcgag gtgccgaaca aaccaacact cagcaaaaag
      841 cacaacaaca acncnaagac agctgatgac gacnacgacg acgacnacna cntccctgcg
      901 anaacggagc aggcaacgca tatagaaccc ngccaccacc ataacngcaa tgggcgacgc
      961 tncnacnccn gcagcgaggc gactgcnact gcnantgcaa ccaacncgng gntgaccnta
     1021 tcngaaatca attcccgcgc cnccatggcg ncgggancat gcgacgtcgg gcccantcnc
     1081 c
//
