LOCUS       sequence-161148-        1060 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of hM4D(Gi) using the CD68 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Grace PM, Strand KA, Galer EL, Urban DJ, Wang X, Baratta MV, 
            Fabisiak TJ, Anderson ND, Cheng K, Greene LI, Berkelhammer D, Zhang 
            Y, Ellis AL, Yin HH, Campeau S, Rice KC, Roth BL, Maier SF, Watkins 
            LR
  TITLE     Morphine paradoxically prolongs neuropathic pain in rats by 
            amplifying spinal NLRP3 inflammasome activation.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jun 14;113(24):E3441-50. doi: 
            10.1073/pnas.1602070113. Epub 2016 May 31.
  PUBMED    27247388
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1602070113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-06-14- 14"; volume: "113"; issue: "24"; pages: 
            "E3441-50"
COMMENT     Sequence Label: pAAV CD68-hM4D(Gi)-mCherry
FEATURES             Location/Qualifiers
     source          1..1060
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      107..116
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcgccctcga tctcgaactc gtggccgttc acggagccct ccatgtgcac cttgaagcgc
       61 atgaactcct tgatgatggc catgttatcc tcctcgccct tgctcaccat ggtggcgacc
      121 ggtggatccc gcctggcagt gccgatgttc cgatactggc acagcagcag gtgccggaag
      181 gtctttttaa aggtggcgtt gcacagagca tagcaggcag ggttgatggt gctgttgacg
      241 tagcagagcc agtagccaat ggaccacacc gtgtcaggga tgcagctctg gcagaaggtg
      301 ttcaccagga ccatgacgtt gtagggcgtc caggtgagga tgaaggccag cagaatggca
      361 aagatcgttc gtgtcacttt gcgctcccgg gccgccatct gccgcttctt gcgcacctgg
      421 ttgcgagcga tgctggcgaa cttgcgggcc acgttggccg cagggcgcat gccagccggc
      481 gtggcaggca caatctcaat ggctgtcaca cactcattgc ctgtctgctt cgtcacaatc
      541 tggatcttgg accatctgga ggctgggttg agggcccgcg gctgcagggg aggggcgggc
      601 atggcgggcg tggtggcctc tgtggtggac agctctgtgg ctgggcgttc cttggtgttc
      661 tgggtggcac tgcctgagct ggactcattg gaagtgtcct tatcagccac ggggcgcggt
      721 ggcggtggca gcgctggcgg gggggcctcc tccagcttgc cattgcgcag ctcctcccgg
      781 gcggcctccc cgggcggggg cttcttgacg ctctgcttca ttagtgggct cttgaggaag
      841 gccagcgtct tggctttctt ctccttcggg ccctcgggcc ggtgcttgtg gactcggctg
      901 cgactggcca gggagatgtg gatgtacagc accgtcatga tgaccacagg caggtagaag
      961 ccagcaatgg ctgtgccaaa ggtcactgct gggttggaca ggaactggat gaagcactgg
     1021 ttgtcgggca ccgtccgctt acccaccaca aactgccaga
//
