LOCUS       sequence-161154-         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-regulated lentiviral shRNA vector. Cre addition causes EGFP to 
            be recombined out of the construct, activating shRNA expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Ventura A, Meissner A, Dillon CP, McManus M, Sharp PA, Van Parijs L,
            Jaenisch R, Jacks T
  TITLE     Cre-lox-regulated conditional RNA interference from transgenes.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Jul 13. 101(28):10380-5.
  PUBMED    15240889
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2004 Jul 13. 101(28):10380-5."
COMMENT     Sequence Label: pSico
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     326..346
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     protein_bind    complement(364..397)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     enhancer        497..800
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
       61 gatccagttt ggttagtacc gggcccgctc tagagatccg acgcgccatc tctaggcccg
      121 cgccggcccc ctcgcacaga cttgtgggag aagctcggct actcccctgc cccggttaat
      181 ttgcatataa tatttcctag taactataga ggcttaatgt gcgataaaag acagataatc
      241 tgttcttttt aatactagct acattttaca tgataggctt ggatttctat aagagataca
      301 aatactaaat tattatttta aaaaacagca caaaaggaaa ctcaccctaa ctgtaaagta
      361 attataactt cgtatagtat aaattatacg aagttataag ccttggtttt ttgaattccg
      421 tattaccgcc atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc
      481 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      541 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      601 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      661 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      721 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      781 tagtcatcgc tattaccatg gtgatgcggt tttggcagta c
//
