LOCUS       sequence-161156-         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  5' entry vector containing human phosphoglycerate kinase (hPGK) 
            promoter for ubiquitous expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: p5E-hPGK
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(167..290)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(409..427)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
       61 ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagag ctgcagggcg
      121 gccgcgataa ttctacaggt cactaatacc atctaagtag ttggttcata gtgactgcat
      181 atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt
      241 gatatttata tcattttacg tttctcgttc aacttttttg tacaaacttg ggcgcatctg
      301 cagaattcca ccacactgga ctagtcgacg aaccggtgga tccccctggg gagagaggtc
      361 ggtgattcgg tcaacgaggg agccgactgc cgacgtgcgc tccggaggct tgcagaatgc
      421 ggaacaccgc gcgggcagga acagggccca cactaccgcc ccacaccccg cctcccgcac
      481 cgccccttcc cggccgctgc tctcggcgcg ccctgctgag cagccgctat tggccacagc
      541 ccatcgcggt cggcgcgctg ccattgctcc ctggcgctgt ccgtctgcga gggtactagt
      601 gagacgtgcg gcttccgttt gtcacgtccg gcacgccgcg aaccgcaagg aaccttcccg
      661 acttaggggc ggagcaggaa gcgtcgccgg ggggcccaca agggtagcgg cgaagatccg
      721 ggtgacgctg cgaacggacg tgaagaatgt gcgagaccca gggtcggcgc cgctgcgtt
//
