LOCUS       sequence-161163-         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Narayana N, Cox S, Shaltiel S, Taylor SS, Xuong N
  TITLE     Crystal structure of a polyhistidine-tagged recombinant catalytic 
            subunit of cAMP-dependent protein kinase complexed with the peptide 
            inhibitor PKI(5-24) and adenosine.
  JOURNAL   Biochemistry. 1997 Apr 15. 36(15):4438-48.
  PUBMED    9109651
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 1997 Apr 15. 36(15):4438-48."
COMMENT     Sequence Label: pET15b PKA Cat
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             6..28
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             74..91
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ataattttgt ttaactttaa gaaggagata taccatgggc agcagccatc atcatcatca
       61 tcacagcagc ggcctggtgc cgcgcggcag ccatatgggc aacgccgccg ccgccaagaa
      121 gggcagcgag caggagagcg tgaaagagtt cctagccaaa gccaaggaag atttcctgaa
      181 aaaatgggag accccttctc agaatacagc ccagttggat cagtttgata gaatcaagac
      241 ccttggcacc ggctcctttg ggcgagtgat gctggtgaag cacaaggaga gtgggaacca
      301 ctacgccatg aagatcttag acaagcagaa ggtggtgaag ctaaagcaga tcgagcacac
      361 tctgaatgag aagcgcatcc tgcaggccgt caacttcccg ttcctggtca aacttgaatt
      421 ctccttcaag gacaactcaa acctgtacat ggtcatggag tatgtagctg gtggcgagat
      481 gttctcccac ctacggcgga ttggaaggtt cagcgagccc catgcccgtt tctacgcggc
      541 gcagatcgtc ctgacctttg agtatctgca ctccctggac ctcatctacc gggacctgaa
      601 gcccgagaat cttctcatcg accagcaggg ctatattcag gtgacagact tcggttttgc
      661 caagcgtgtg aaaggccgta cttggacctt gtgtgggacc cctgagtact tggcccccga
      721 gattatcctg agcaaaggct acaacaaggc tgtggactgg tgggctctcg gagtcctcat
      781 ctacgagatg gctgctggtt acccaccctt cttcgctgac cagcctatcc agatctatga
      841 gaa
//
