LOCUS       sequence-161167-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive CcaS and CcaR with gfpmut3 under the cpcG2-Delta-59 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Milias-Argeitis A, Rullan M, Aoki SK, Buchmann P, Khammash M
  TITLE     Automated optogenetic feedback control for precise and robust 
            regulation of gene expression and cell growth.
  JOURNAL   Nat Commun. 2016 Aug 26;7:12546. doi: 10.1038/ncomms12546.
  PUBMED    27562138
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-08-26"; volume: "7:12546. doi"; pages: " 
            10.1038/ncomms12546"
COMMENT     Sequence Label: pSKA413
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     576..604
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tgggatcttt cgaaagggca gattgtgtgg acaggtaatg gttgtctggt aaaaggacag
       61 ggccatcgcc aattggagta ttttgttgat aatggtctgc tagttgaacg cttccatctt
      121 caatgttgtg tctaattttg aagttaactt tgattccatt cttttgtttg tctgccatga
      181 tgtatacatt gtgtgagtta tagttgtatt ccaatttgtg tccaagaatg tttccatctt
      241 ctttaaaatc aatacctttt aactcgattc tattaacaag ggtatcacct tcaaacttga
      301 cttcagcacg tgtcttgtag ttcccgtcat ctttgaaaaa tatagttctt tcctgtacat
      361 aaccttcggg catggcactc ttgaaaaagt catgctgttt catatgatct gggtatctcg
      421 caaagcattg aacaccataa ccgaaagtag tgacaagtgt tggccatgga acaggtagtt
      481 ttccagtagt gcaaataaat ttaagggtaa gttttccgta tgttgcatca ccttcaccct
      541 ctccactgac agaaaatttg tgcccattaa catcaccatc taattcaaca agaattggga
      601 caactccagt gaaaagttct tctcctttac gcattatatg gtgccttacg tgcctgtccc
      661 ttagataaag ttagaatgcg atcctaacaa agtaaaattg aagaaaaggg ggagaaaatc
      721 gtaaagaaat cggaaagtta gctgatgcga ctaaaactgt tacatttcgc aacatgaatt
      781 aacccagacc tgttcacccc ttcaggtaag ctgaggttga tagagaaaag caggatgggc
      841 ta
//
