LOCUS       sequence-161168-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  5' entry vector containing the EF1a/B-globin fused to zebrafish 
            b-actin 2 enhancer/promoter for strong semi-ubiquitous expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: p5E-EF1a/b-actin
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(67..190)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        191..209
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     246..262
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     246..262
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 cgcggccgcc ctgcagctct agagctcgaa ttctacaggt cactaatacc atctaagtag
       61 ttggttcata gtgactgcat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc
      121 aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc aacttttttg
      181 tacaaacttg aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggtggcg
      241 gccgctctag aactagtgga tccgtcgagg aattctttgc caaaatgatg agacagcaca
      301 acaaccagca cgttgcccag gagctgtagg aaagagaaga aggcatgaac atggttagca
      361 gaggggcccg gtttggactc agagtatttt atcctcatct caaacagtgt atatcattgt
      421 aaccataaag agaaaggcag gatgatgacc agggtgtagt tgtttctacc aataagaata
      481 tttccacgcc agccagaatt tatatgcaga aatattctac cttatcattt aattataaca
      541 attgttctct aaaactgtgc tgaagtacaa tataatatac cctgattgcc ttgaaaaaaa
      601 agtgattaga gaaagtactt acaatctgac aaataaacaa aagtgaattt aaaaattcgt
      661 tacaaatgca agctaaagtt taacgaaaaa gttacagaaa atgaaaagaa aataagagga
      721 gacaatggtt gtcaacagag tagaaagtga aagaaacaaa attatcatga gggtccatgg
      781 tgatacaagg gacatcttcc
//
