LOCUS       sequence-161181-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector targeting the Clover gene to the GAPDH locus of human cells. 
            Clover is expressed from a 2A sequence at the C terminus of GAPDH. 
            Puromycin resistance is encoded by the Muro gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris
            FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E,
            Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ,
            Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG
  TITLE     GAPTrap: A Simple Expression System for Pluripotent Stem Cells and 
            Their Derivatives.
  JOURNAL   Stem Cell Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015.
  PUBMED    27594589
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015."
COMMENT     Sequence Label: pGAPTrap-Clover-Muro
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..84
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(709..726)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    715..826
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctgtacaagg aagggggcgg ctctggcggg gagggaagag gaagtcttct aacatgcggt
       61 gacgtggagg aaaaccccgg gcccatcgaa atggcaagac tgaccgaata caagcctacc
      121 gtgagactgg caacaagaga tgatgtccca agagccgtgc gaaccctggc agcagcattc
      181 gcagactacc cagccacaag acacactgtg gaccccgata ggcatatcga gagagtgacc
      241 gagctgcagg aactgtttct gacacgggtg ggcctggata ttggaaaggt ctgggtggca
      301 gatgatgggg ctgctgtggc cgtctggaca accccagagt ctgtggaagc tggagctgtc
      361 ttcgctgaga tcggccctcg aatggcagaa ctgtctggct ccaggctggc agcacagcag
      421 cagatggagg gactgctggc accacacaga cctaaggaac cagcttggtt cctggcaact
      481 gtgggggtct ctcccgacca tcagggcaaa ggactgggga gtgccgtggt cctgcctgga
      541 gtggaggctg cagaaagagc tggagtccca gcttttctgg agacatctgc cccccggaac
      601 ctgcctttct atgaacgcct gggctttact gtgaccgctg acgtggaggt gcccgaaggc
      661 ccccgaactt ggtgcatgac ccgaaagcca ggagcataac ctgatcagcc tcgactgtgc
      721 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
      781 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
      841 ggtgtcattc tattctgggg
//
