LOCUS       sequence-161182-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector targeting the mtagBFP2 gene to the GAPDH locus of human 
            cells. BFP is expressed from a 2A sequence at the C terminus of 
            GAPDH. Puromycin resistance is encoded by the Muro gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris
            FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E,
            Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ,
            Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG
  TITLE     GAPTrap: A Simple Expression System for Pluripotent Stem Cells and 
            Their Derivatives.
  JOURNAL   Stem Cell Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015.
  PUBMED    27594589
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015."
COMMENT     Sequence Label: pGAPTrap-mtagBFP2-IRESMuro
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..56)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 atcgggccag gattctcctc cacgtcaccg catgttagaa gacttcctct gccctctcct
       61 ccggacccgc cgccacgcgc cattccctcc ttggaggcca tgtgggccat gaggtccacc
      121 accctgttgc tgtagccaaa ttcgttgtca tacctagaag atgaaaagag ttgtcagggc
      181 cctttttctg agccagccac cagagggcgc cagaccctgc actttttaag agccagtctc
      241 tggccccagc cacataccag gaaatgagct tgacaaagtg gtcgttgagg gcaatgccag
      301 ccccagcgtc aaaggtggag gagtgggtgt cgctgttgaa gtcagaggag accacctggt
      361 gctcagtgta gcccaggatg cccttgaggg ggccctccga cgcctgcttc accaccttct
      421 tgatgtcatc atatttggca ggtttttcta gacggcaggt caggtccacc actgacacgt
      481 tggcagtggg gacacggaag gccatgccag tgagcttccc gttcagctca gggatgacct
      541 tgcccacagc cttggcagcg ccagtagagg cagggatgat gttctggaga gccccgcggc
      601 catcacgcca cagtttcccg gaggggccat ccacagtctt ctgggtggca gtgatggcat
      661 ggactgtggt ctgcaaaagg agtgaggccc tgcagcgtac tccccacatc acccctctac
      721 ctccctcccc accttgaaag gaaattatgg gaaagccagt ccccagaacc cccaacccca
      781 gggttgcacg cagccccggc agggaggagc cagtcttgga tgagaaaggt gggagcctca
      841 gtcccattcc ccagctctca taccatgagt ccttccacga taccaaagtt gtcatg
//
