LOCUS       sequence-161183-         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector targeting the mtagBFP2 gene to the GAPDH locus of human 
            cells. BFP is expressed from a 2A sequence at the C terminus of 
            GAPDH. Puromycin resistance is encoded by the Muro gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris
            FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E,
            Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ,
            Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG
  TITLE     GAPTrap: A Simple Expression System for Pluripotent Stem Cells and 
            Their Derivatives.
  JOURNAL   Stem Cell Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015.
  PUBMED    27594589
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015."
COMMENT     Sequence Label: pGAPTrap-mtagBFP2-IRESMuro
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ccattgtatg ggatctgatc tggggcctcg gtacacatgc tttacatgtg tttagtcgag
       61 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
      121 atgataatat ggccacaacc atggcgcgta tggcaagact gaccgaatac aagcctaccg
      181 tgagactggc aacaagagat gatgtcccaa gagccgtgcg aaccctggca gcagcattcg
      241 cagactaccc agccacaaga cacactgtgg accccgatag gcatatcgag agagtgaccg
      301 agctgcagga actgtttctg acacgggtgg gcctggatat tggaaaggtc tgggtggcag
      361 atgatggggc tgctgtggcc gtctggacaa ccccagagtc tgtggaagct ggagctgtct
      421 tcgctgagat cggccctcga atggcagaac tgtctggctc caggctggca gcacagcagc
      481 agatggaggg actgctggca ccacacagac ctaaggaacc agcttggttc ctggcaactg
      541 tgggggtctc tcccgaccat cagggcaaag gactggggag tgccgtggtc ctgcctggag
      601 tggaggctgc agaaagagct ggagtcccag cttttctgga gacatctgcc ccccggaacc
      661 tgcctttcta tgaacgcctg ggctttactg tgaccgctga cgtggaggtg cccgaaggcc
      721 cccgaacttg gtgcatgacc cgaaagccag gagcataaat ctagacgcgt atcgataagc
      781 ttagatcgag cacaagagga agagagagac cctcactgct ggggagtccc tgccacactc
      841 agtcccccac cacactgaat ctcccctcct cacagttgcc atgtagaccc cttgaagagg
      901 ggaggggcct a
//
