LOCUS       sequence-161184-         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Base vector targeting genes to the GAPDH locus of human cells. 
            Inserted genes are expressed from a 2A sequence at the C terminus of
            GAPDH. Puromycin resistance is encoded by the Muro gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris
            FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E,
            Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ,
            Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG
  TITLE     GAPTrap: A Simple Expression System for Pluripotent Stem Cells and 
            Their Derivatives.
  JOURNAL   Stem Cell Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015.
  PUBMED    27594589
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015."
COMMENT     Sequence Label: pGAPTrap-IRESMuro
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtaccccatt gtatgggatc tgatctgggg cctcggtaca catgctttac atgtgtttag
       61 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
      121 acacgatgat aatatggcca caaccatggc gcgtatggca agactgaccg aatacaagcc
      181 taccgtgaga ctggcaacaa gagatgatgt cccaagagcc gtgcgaaccc tggcagcagc
      241 attcgcagac tacccagcca caagacacac tgtggacccc gataggcata tcgagagagt
      301 gaccgagctg caggaactgt ttctgacacg ggtgggcctg gatattggaa aggtctgggt
      361 ggcagatgat ggggctgctg tggccgtctg gacaacccca gagtctgtgg aagctggagc
      421 tgtcttcgct gagatcggcc ctcgaatggc agaactgtct ggctccaggc tggcagcaca
      481 gcagcagatg gagggactgc tggcaccaca cagacctaag gaaccagctt ggttcctggc
      541 aactgtgggg gtctctcccg accatcaggg caaaggactg gggagtgccg tggtcctgcc
      601 tggagtggag gctgcagaaa gagctggagt cccagctttt ctggagacat ctgccccccg
      661 gaacctgcct ttctatgaac gcctgggctt tactgtgacc gctgacgtgg aggtgcccga
      721 aggcccccga acttggtgca tgacccgaaa gccaggagca taaatctaga cgcgtatcga
      781 taagcttaga tcgagcacaa gaggaagaga gagaccctca ctgctgggga gtccctgcca
      841 cactcagtcc cccaccacac tgaatctccc ctcctcacag ttgccatgta gaccccttga
      901 agaggggagg ggccta
//
