LOCUS       sequence-161185-         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Base vector targeting genes to the GAPDH locus of human cells. 
            Inserted genes are expressed from a 2A sequence at the C terminus of
            GAPDH. Puromycin resistance is encoded by the Muro gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris
            FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E,
            Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ,
            Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG
  TITLE     GAPTrap: A Simple Expression System for Pluripotent Stem Cells and 
            Their Derivatives.
  JOURNAL   Stem Cell Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015.
  PUBMED    27594589
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015."
COMMENT     Sequence Label: pGAPTrap-IRESMuro
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(89..142)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 aacgcacacc ggccttattc caagcggctt cggccagtaa cgttaggggg gggggaggga
       61 gaggggcgga tccgtcgact tggcgcgccg ggccaggatt ctcctccacg tcaccgcatg
      121 ttagaagact tcctctgccc tctcctccgg acccgccgcc acgcgccatt ccctccttgg
      181 aggccatgtg ggccatgagg tccaccaccc tgttgctgta gccaaattcg ttgtcatacc
      241 tagaagatga aaagagttgt cagggccctt tttctgagcc agccaccaga gggcgccaga
      301 ccctgcactt tttaagagcc agtctctggc cccagccaca taccaggaaa tgagcttgac
      361 aaagtggtcg ttgagggcaa tgccagcccc agcgtcaaag gtggaggagt gggtgtcgct
      421 gttgaagtca gaggagacca cctggtgctc agtgtagccc aggatgccct tgagggggcc
      481 ctccgacgcc tgcttcacca ccttcttgat gtcatcatat ttggcaggtt tttctagacg
      541 gcaggtcagg tccaccactg acacgttggc agtggggaca cggaaggcca tgccagtgag
      601 cttcccgttc agctcaggga tgaccttgcc cacagccttg gcagcgccag tagaggcagg
      661 gatgatgttc tggagagccc cgcggccatc acgccacagt ttcccggagg ggccatccac
      721 agtcttctgg gtggcagtga tggcatggac tgtggtctgc aaaaggagtg aggccctgca
      781 gcgtactccc cacatcaccc ctctacctcc ctccccacct tgaaaggaaa ttatgggaaa
      841 gccagtcccc agaaccccca accccagggt tgcacgcagc ccc
//
