LOCUS       sequence-161187-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Base vector targeting genes to the GAPDH locus of human cells. 
            Inserted genes are expressed from a 2A sequence at the C terminus of
            GAPDH. Puromycin resistance is encoded by the Muro gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris
            FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E,
            Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ,
            Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG
  TITLE     GAPTrap: A Simple Expression System for Pluripotent Stem Cells and 
            Their Derivatives.
  JOURNAL   Stem Cell Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015.
  PUBMED    27594589
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015."
COMMENT     Sequence Label: pGAPTrap-IRESMuro
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aggtcggagt caacgggtga gttcgcgggt ggctgggggg ccctgggctg cgaccgcccc
       61 cgaaccgcgt ctacgagcct tgcgggctcc gggtctttgc agtcgtatgg gggcagggta
      121 gctgttcccc gcaaggagag ctcaaggtca gcgctcggac ctggcggagc cccgcaccca
      181 ggctgtggcg ccctgtgcag ctccgccctt gcggcgccat ctgcccggag cctccttccc
      241 ctagtcccca gaaacaggag gtccctactc ccgcccgaga tcccgacccg gacccctagg
      301 tgggggacgc tttctttcct ttcgcgctct gcggggtcac gtgtcgcaga ggagcccctc
      361 ccccacggcc tccggcaccg caggccccgg gatgctagtg cgcagcgggt gcatccctgt
      421 ccggatgctg cgcctgcggt agagcggccg ccatgttgca accgggaagg aaatgaatgg
      481 gcagccgtta ggaaagcctg ccggtgacta accctgcgct cctgcctcga tgggtggagt
      541 cgcgtgtggc ggggaagtca ggtggagcga ggctagctgg cccgatttct cctccgggtg
      601 atgcttttcc tagattattc tctggtaaat caaagaagtg ggtttatgga ggtcctcttg
      661 tgtcccctcc ccgcagaggt gtggtggctg tggcatggtg ccaagccggg agaagctgag
      721 tcatgggtag ttggaagagg acatttccac cgcaaaatgg cccctctggt ggtggcccct
      781 tcctgcagcg ccggctcacc tcacggcccc gcccttcccc tgccagccta gcgttgaccc
      841 gaccccaaag gccaggctgt aaatgtcacc gggaggattg ggtgtctggg cgcctcgggg
      901 aacctgccct tctccccatt ccgtcttccg
//
