LOCUS       sequence-161190-         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector targeting the mTagtandem tomato gene to the GAPDH locus of 
            human cells. It is expressed from a 2A sequence at the C terminus of
            GAPDH. Puromycin resistance is encoded by the Muro gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris
            FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E,
            Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ,
            Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG
  TITLE     GAPTrap: A Simple Expression System for Pluripotent Stem Cells and 
            Their Derivatives.
  JOURNAL   Stem Cell Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015.
  PUBMED    27594589
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015."
COMMENT     Sequence Label: pGAPTrap-mtagtdTom_IRESMuro
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(92..115)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(290..309)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     559..577
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(711..764)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 ctgccgctgc cggtgctgcc ggtgccatgc cccaggaaca ggtggtggcg gccctcggag
       61 cgctcgtact gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc cagcttggtg
      121 tccacgtagt agtagccggg cagttgcacg ggcttcttgg ccatgtagat ggtcttgaac
      181 tccaccaggt agtggccgcc gtccttcagc ttcagggcct ggtggatctc gcccttcagc
      241 acgccgtcgc gggggtacag gcgctcggtg gaggcctccc agcccatggt cttcttctgc
      301 attacggggc cgtcgggggg gaagttggtg ccgcgcatct tcaccttgta gatcagcgtg
      361 ccgtcctgca gggaggagtc ctgggtcacg gtcaccagac cgccgtcctc gaagttcatc
      421 acgcgctccc acttgaagcc ctcggggaag gacagcttct tgtaatcggg gatgtcggcg
      481 gggtgcttca cgtacgcctt ggagccgtac atgaactggg gggacaggat gtcccaggcg
      541 aagggcaggg ggccgccctt ggtcaccttc agcttggcgg tctgggtgcc ctcgtagggg
      601 cggccctcgc cctcgccctc gatctcgaac tcgtggccgt tcatggagcc ctccatgcgc
      661 accttgaagc gcatgaactc tttgatgacc tcctcgccct tgctcaccat cgggccagga
      721 ttctcctcca cgtcaccgca tgttagaaga cttcctctgc cctctcctcc ggacccgccg
      781 ccacgcgcca ttccctcctt ggaggccatg tgggccatga ggtccaccac cctgttgctg
      841 tagccaaatt cgttgtcata cctagaagat gaaaagagtt gtc
//
