LOCUS       sequence-161197-         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  5' entry vector containing the zebrafish krt5 promoter for 
            epidermis-specific expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Akerberg AA, Stewart S, Stankunas K
  TITLE     Spatial and temporal control of transgene expression in zebrafish.
  JOURNAL   PLoS One. 2014 Mar 18;9(3):e92217. doi: 
            10.1371/journal.pone.0092217. eCollection 2014.
  PUBMED    24643048
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-03-18"; volume: "9(3):e92217. doi"; pages: " 
            10.1371/journal.pone.0092217. eCollection 2014"
COMMENT     Sequence Label: p5E-krt5
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(68..191)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        192..210
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     247..263
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     247..263
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tcgcggccgc cctgcagctc tagagctcga attctacagg tcactaatac catctaagta
       61 gttggttcat agtgactgca tatgttgtgt tttacagtat tatgtagtct gttttttatg
      121 caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt caactttttt
      181 gtacaaactt gaattaaccc tcactaaagg gaacaaaagc tggagctcca ccgcggtggc
      241 ggccgctcta gaactagtgg atccctgtcg tctacgaggg ggtgaggatc agaaaaagag
      301 caggatgaga cagagatctg gagcaggaga gctcactaag aggaaagtaa aatccctctg
      361 agtgccctct tcaagcagtg cagtggcttt tatacctctt acctcatggg aggggctcac
      421 ctggccaatc ccctttctgc ctcaggcttc tgtcctgcct ttttccctgt ccaagtgata
      481 cacacgccca tcttgctggg ctggtatggg agtatgttca gcattggagg ccaggccaca
      541 ccctgcctta gatgcacatg catactcacg taataaaaca gagctattaa gagagaaaca
      601 gagtagaaaa acaaggttaa actccacaca cccttgtatg tactacgact cagaatatac
      661 aagttctctg taatttaaag cccctcttat ttacataacc agatggcatt ctccaaatgc
      721 aaaccataca actccatgaa tattttatga ttcatgcagg tatggggccc tcagtttgct
      781 ttaagtgcaa tgcaggagta ttaaatagg
//
