LOCUS       sequence-161199-         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral shRNA vector for inducible knockdown of human ID2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
  TITLE     Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor 
            Sequestration in Triple-Negative Breast Cancer.
  JOURNAL   Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 
            10.1016/j.devcel.2017.10.027.
  PUBMED    29161592
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.10"; journalName: "Dev Cell"; date: 
            "2017-11-20- 20"; volume: "43"; issue: "4"; pages: "418-435.e13"
COMMENT     Sequence Label: TET-pLKO.1 PURO shID2 #1
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(43..137)
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    43..61
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    71..89
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(107..126)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     CDS             complement(426..470)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 atgtcaaatc tgcagatttg acattaactc agaaggccgg tgtctctatc actgataggg
       61 aacttataag tctctatcac tgatagggat ttcacgttta tggtgatttc ccagaacaca
      121 tagcgacatg caaatattgc ggccgctcga ggctagtctc gtgatcgata ccgtcgagat
      181 ccgttcacta atcgaatgga tctgtctctg tctctctctc caccttcttc ttctattcct
      241 tcgggcctgt cgggtcccct cggggttggg aggtgggtct gaaacgataa tggtgaatat
      301 ccctgcctaa ctctattcac tatagaaagt acagcaaaaa ctattcttaa acctaccaag
      361 cctcctacta tcattatgaa taattttata taccacagcc aatttgttat gttaaaccaa
      421 ttccacaaac ttgcccattt atctaattcc aataattctt gttcattctt ttcttgctgg
      481 ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt aattgttaat ttctctgtcc
      541 cactccatcc aggtcgtgtg attccaaatc tgttccagag atttattact ccaactagca
      601 ttccaaggca cagcagtggt gcaaatgagt tttccagagc aaccccaaat ccccaggagc
      661 tgttgatcct ttaggtatct ttccacagcc aggattcttg cctggagctg cttgatgccc
      721 cagactgtga gttgcaacag atgctgttgc gcctcaatag ccctcagcaa attgttctgc
      781 tgctgcacta taccagacaa taattg
//
