LOCUS       sequence-161200-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  5'entry vector containing human ubiquitin C promoter followed by 
            intronically-expressed eSIBR cassette for ubiquitous expression, 
            RNAi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: p5E-Ui4-eSIBR
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..106
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        129..528
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
ORIGIN
        1 atggagctcc aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt
       61 gataagcaat gcttttttat aatgccaact ttgtatagaa aagttgggcc ggcccttcgg
      121 cgcaaactgg cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc tcctcacggc
      181 gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct gatccttccg cccggacgct
      241 caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc agcagaagga
      301 cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag agagcggaac
      361 aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt ggggcggtga
      421 acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt cgcagccggg
      481 atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct
      541 gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga
      601 gagaccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac tgggggttgg
      661 ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa tagaaccttc gctaatgcgg
      721 gaaagctctt attcgggtga gatgggctgg ggcaccatct ggggaccctg acgtgaagtt
      781 tgtcactgac tggagaactc ggtttgtcgt ctgttgcggg ggcggcagtt atggcggtgc
      841 cgttgg
//
