LOCUS       sequence-161201-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TRP1-ARS1-8xLacO (TALO8) minichromosome system for histone 
            purification in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Unnikrishnan A, Gafken PR, Tsukiyama T
  TITLE     Dynamic changes in histone acetylation regulate origins of DNA 
            replication.
  JOURNAL   Nat Struct Mol Biol. 2010 Apr;17(4):430-7. doi: 10.1038/nsmb.1780. 
            Epub 2010 Mar 14.
  PUBMED    20228802
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.1780";
            journalName: "Nat Struct Mol Biol"; date: "2010-04"; volume: "17"; 
            issue: "4"; pages: "430-7"
COMMENT     Sequence Label: pUC-TALO8
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        42..143
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             144..818
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
ORIGIN
        1 cctgcaggtc gactctagag gatccccggg taccgagctc gaattcggtc gaaaaaagaa
       61 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta
      121 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat
      181 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg
      241 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg
      301 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact
      361 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg
      421 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg
      481 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag
      541 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg
      601 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct
      661 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa
      721 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta
      781 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat
      841 tt
//
