LOCUS       sequence-161207-         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral shRNA vector for inducible knockdown of mouse Id2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
  TITLE     Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor 
            Sequestration in Triple-Negative Breast Cancer.
  JOURNAL   Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 
            10.1016/j.devcel.2017.10.027.
  PUBMED    29161592
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.10"; journalName: "Dev Cell"; date: 
            "2017-11-20- 20"; volume: "43"; issue: "4"; pages: "418-435.e13"
COMMENT     Sequence Label: TET-pLKO.1 PURO shId2 #2
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(41..135)
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    41..59
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    69..87
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(105..124)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     CDS             complement(424..468)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 atgatagctg cagctatcat tcgacataag ctcaccggtg tctctatcac tgatagggaa
       61 cttataagtc tctatcactg atagggattt cacgtttatg gtgatttccc agaacacata
      121 gcgacatgca aatattgcgg ccgctcgagg ctagtctcgt gatcgatacc gtcgagatcc
      181 gttcactaat cgaatggatc tgtctctgtc tctctctcca ccttcttctt ctattccttc
      241 gggcctgtcg ggtcccctcg gggttgggag gtgggtctga aacgataatg gtgaatatcc
      301 ctgcctaact ctattcacta tagaaagtac agcaaaaact attcttaaac ctaccaagcc
      361 tcctactatc attatgaata attttatata ccacagccaa tttgttatgt taaaccaatt
      421 ccacaaactt gcccatttat ctaattccaa taattcttgt tcattctttt cttgctggtt
      481 ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca
      541 ctccatccag gtcgtgtgat tccaaatctg ttccagagat ttattactcc aactagcatt
      601 ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc ccaggagctg
      661 ttgatccttt aggtatcttt ccacagccag gattcttgcc tggagctgct tgatgcccca
      721 gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc ctcagcaa
//
