LOCUS       sequence-161210-         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 and contains guide RNA expression cassette with 
            BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 
            marker for yeast transformation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  TITLE     Patricia Wittkopp yeast CRISPR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pML104-HygMx4
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      112..121
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aattctactt ttatagttag tctttttttt agttttaaaa caccaagaac ttagtttcga
       61 ataaacacac ataaacaaac aaaagatctg atatggatcg aattagatct cgccaccatg
      121 gacaagaagt attctatcgg actggacatc gggactaata gcgtcgggtg ggccgtcatc
      181 actgacgagt acaaggtgcc ctctaagaag ttcaaggtgc tcgggaacac cgaccggcat
      241 tccatcaaga aaaatctgat cggagctctc ctctttgatt caggggagac cgctgaagca
      301 acccgcctca agcggactgc tagacggcgg tacaccagga ggaagaaccg gatttgttac
      361 cttcaagaga tattctccaa cgaaatggca aaggtcgacg acagcttctt ccataggctg
      421 gaagaatcat tcctcgtgga agaggataag aagcatgaac ggcatcccat cttcggtaat
      481 atcgtcgacg aggtggccta tcacgagaaa tacccaacca tctaccatct tcgcaaaaag
      541 ctggtggact caaccgacaa ggcagacctc cggcttatct acctggccct ggcccacatg
      601 attaagttca gaggccactt cctgatcgag ggcgacctca atcctgacaa tagcgatgtg
      661 gataaactgt tcatccagct ggtgcagact tacaaccagc tctttgaaga gaaccccatc
      721 aatgcaagcg gagtcgatgc caaggccatt ctgtcagccc ggctgtcaaa gagccgcaga
      781 cttgagaatc ttatcgctca gctgccgggt gaaaagaaaa atggactgtt cgggaacctg
      841 attgctcttt cacttgggct gactcccaat ttcaagtcta atttcgacct ggcagaggat
      901 gccaagctgc aactgtccaa ggacacctat gatgacgatc tcgacaacct c
//
