LOCUS       sequence-161212-         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  5'entry vector containing human ubiquitin C promoter followed by 
            intronically-expressed eSIBR cassette for ubiquitous expression, 
            RNAi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: p5E-Ui4-eSIBR
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(167..290)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(320..339)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
       61 ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagag ctgcagggcg
      121 gccgcgataa ttctacaggt cactaatacc atctaagtag ttggttcata gtgactgcat
      181 atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt
      241 gatatttata tcattttacg tttctcgttc aacttttttg tacaaacttg ggcgcggatc
      301 cttaagcttg atgagacagc acaataacca gcacgttgcc caggagctgt aggaaaaaga
      361 agaaggcatg aacatggtta gcagaggggc ccggtttgga ctcagagtat tttatcctca
      421 tctcaaacag tgtatatcat tgtaaccata aagagaaagg caggatgatg accaggatgt
      481 agttgtttct accaataaga atatttccac gccagccaga atttatatgc agaaatattc
      541 taccttatca tttaattata acaattgttc tctaaaactg tgctgaagta caatataata
      601 taccctgatt gtctagaggc tcgagttgtc atcctcccac ggtggccatt tgttccatgt
      661 gagtgctgat aaagggcctt gtgtcctgtg tcttccagat cttgaagaca acagcataca
      721 gcccaaagca agcctccagt cgactttgaa ttcgaatcga tgggatctta caatctgaca
      781 aataaacaaa agtgaattta aaaattcgtt acaaatgcaa gctaaagttt aacga
//
